Article
Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome.
BMC biotechnology - 17 Sept 2007
Cox Mandy M, Layton Sherryll L, Jiang Tieshan, Cole Kim, Hargis Billy M, Berghman Luc R, Bottje Walter G, Kwon Young Min
Abstract excerpt
BACKGROUND: A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for vaccine development. Most methods use a multi-step approach that requires cloning and/or designing repeat sequences to facilitate homologous recombination. We...
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