Article
Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake.
FEMS yeast research - 1 Sept 2018
Verhoeven Maarten D, Bracher Jasmine M, Nijland Jeroen G, Bouwknegt Jonna, Daran Jean-Marc G, Driessen Arnold J M, van Maris Antonius J A, Pronk Jack T
Abstract excerpt
Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus plantaruml-arabinose pathway and the Penicillium chrysogenum transporter PcAraT. This strain, which showed a growth rate of 0.26 h-1 on l-arabinose in aerobic batch cultures, was subsequently evolved for anaerobic growth on l-arabinose in the presence of d-glucose...
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