Article
Automated IS6110-based fingerprinting of Mycobacterium tuberculosis: Reaching unprecedented discriminatory power and versatility.
PloS one - 1 Jan 2018
Dekhil Naira, Skhairia Mohamed Amine, Mhenni Besma, Ben Fradj Saloua, Warren Rob, Mardassi Helmi
Abstract excerpt
BACKGROUND: Several technical hurdles and limitations have restricted the use of IS6110 restriction fragment length polymorphism (IS6110 RFLP), the most effective typing method for detecting recent tuberculosis (TB) transmission events. This has prompted us to conceive an alternative modality, IS6110-5'3'FP, a plasmid-based cloning approach coupled to a single PCR amplification of differentially labeled 5' and 3'...
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