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Article

PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers.

Journal of clinical microbiology - 1 Jan 2004

Kotłowski Roman, Shamputa Isdore Chola, El Aila Nabil Abdullah, Sajduda Anna, Rigouts Leen, van Deun Armand, Portaels Françoise

Abstract excerpt

In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5'-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5'-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of M. tuberculosis isolates.

Topics

DNA Fingerprinting
DNA Primers
DNA Transposable Elements
Genotype
Mycobacterium tuberculosis
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 14715782
DOI: 10.1128/JCM.42.1.372-377.2004
PMCID: PMC321654

Genes

GCG

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