Article
Rapid, amplification-based fingerprinting of Mycobacterium tuberculosis.
Journal of general microbiology - 1 Jul 1993
Plikaytis B B, Crawford J T, Woodley C L, Butler W R, Eisenach K D, Cave M D, Shinnick T M
Abstract excerpt
Insertion element IS6110 occurs in multiple copies throughout the Mycobacterium tuberculosis genome, and the variability of its insertion sites is the basis for the IS6110 restriction fragment length polymorphism (RFLP) method for typing. We describe a novel gene amplification method to assess the variability of the location of IS6110. A unilateral-nested polymerase chain reaction and hybridization procedure was...
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