Article
Rapid, amplification-based fingerprinting of Mycobacterium tuberculosis.
Journal of general microbiology - 1 Jul 1993
Plikaytis B B, Crawford J T, Woodley C L, Butler W R, Eisenach K D, Cave M D, Shinnick T M
Abstract excerpt
Insertion element IS6110 occurs in multiple copies throughout the Mycobacterium tuberculosis genome, and the variability of its insertion sites is the basis for the IS6110 restriction fragment length polymorphism (RFLP) method for typing. We describe a novel gene amplification method to assess th...
Topics
- Bacterial Typing Techniques
- Base Sequence
- Chromosome Mapping
- DNA Fingerprinting
- DNA Transposable Elements
- Disease Outbreaks
- Drug Resistance, Microbial
- Gene Amplification
- Genetic Variation
- Humans
- Molecular Sequence Data
- Mycobacterium tuberculosis
- Oligonucleotides
- Polymorphism, Restriction Fragment Length
- Repetitive Sequences, Nucleic Acid
- Sputum
- Tuberculosis