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[Establishment and application of a standard IS6110-RFLP method in the study of molecular genotyping analysis on Mycobacterium tuberculosis].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Aug 2008

Liu Jing-hua, Kremer Kristin, Pourcel Christine, Mulder Arnout, Liu Zhi-guang, Zhao Xiu-qin, Wan Kang-lin

Abstract excerpt

OBJECTIVE: To develop a standardized IS6110-restriction fragment length polymorphism (RFLP) method, used for evaluating the capacity of genotyping. METHODS: IS6110-RFLP of 78 Mycobacterium (M.) tuberculosis strains were studied by bio-molecular techniques including DNA isolation, PCR, restriction endonuclease enzyme analysis, southern blotting, agarose gel electrophoresis, together with data analysis by software...

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PMID: 19103118

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