Article
Use of pulsed-field gel electrophoresis for molecular epidemiologic and population genetic studies of Mycobacterium tuberculosis.
Journal of clinical microbiology - 1 Jun 1999
Singh S P, Salamon H, Lahti C J, Farid-Moyer M, Small P M
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE) is a powerful molecular biology technique which has provided important insights into the epidemiology and population biology of many pathogens. However, few studies have used PFGE for the molecular epidemiology of Mycobacterium tuberculosis. A laboratory protocol was developed to determine the typeability, stability, and reproducibility of PFGE typing of M. tuberculosis....
Topics
- DNA Transposable Elements
- DNA, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Genetic Variation
- Humans
- Molecular Epidemiology
- Mycobacterium tuberculosis
- Polymorphism, Restriction Fragment Length
- Restriction Mapping
- San Francisco
- Tuberculosis