Article
Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units.
Journal of clinical microbiology - 1 Oct 2001
Supply P, Lesjean S, Savine E, Kremer K, van Soolingen D, Locht C
Abstract excerpt
Large-scale genotyping of Mycobacterium tuberculosis is especially challenging, as the current typing methods are labor-intensive and the results are difficult to compare among laboratories. Here, automated typing based on variable-number tandem repeats (VNTRs) of genetic elements named mycobacterial interspersed repetitive units (MIRUs) in 12 mammalian minisatellite-like loci of M. tuberculosis is presented....
Topics
- Automation
- Bacterial Typing Techniques
- Genotype
- Global Health
- Humans
- Minisatellite Repeats
- Mycobacterium tuberculosis
- Polymerase Chain Reaction
- Tuberculosis, Pulmonary