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Article

Pyrosequencing-based quantitative measurement of CALR mutation allele burdens and their clinical implications in patients with myeloproliferative neoplasms.

Clinica chimica acta; international journal of clinical chemistry - 1 Aug 2018

Oh Yejin, Song Ik-Chan, Kim Jimyung, Kwon Gye Cheol, Koo Sun Hoe, Kim Seon Young

Abstract excerpt

BACKGROUND: We developed a pyrosequencing-based method for the quantification of CALR mutations and compared the results using Sanger sequencing, fragment length analysis (FLA), digital-droplet PCR (ddPCR), and next-generation sequencing (NGS). METHODS: Method validation studies were performed using cloned plasmid controls. Samples from 24 patients with myeloproliferative neoplasms were evaluated. RESULTS: Among...

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Source

Identifiers

PMID: 29727699
DOI: 10.1016/j.cca.2018.05.001

Genes

CALR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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