Clonal Lineage and Gene Diversity Analysis of Paired Antibody Heavy and Light Chains.

doi:10.1101/pdb.prot108628

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Article

Clonal Lineage and Gene Diversity Analysis of Paired Antibody Heavy and Light Chains.

Cold Spring Harbor protocols - 2 Jan 2025

Fahad Ahmed S, Gutiérrez-Gonzalez Matías F, Madan Bharat, DeKosky Brandon J

Abstract excerpt

Antibodies consist of unique variable heavy (VH) and variable light (VL) chains, and both are required to fully characterize an antibody. Methods to detect paired heavy and light chain variable regions (VH:VL) using high-throughput sequencing (HTS) have recently enabled large-scale analysis of complete functional antibody responses. Here, we describe an HTS computational pipeline to analyze paired VH:VL antibody...

Topics

Immunoglobulin Light Chains
Immunoglobulin Heavy Chains
High-Throughput Nucleotide Sequencing
Genetic Variation
Computational Biology
Immunoglobulin Variable Region
Humans

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PMID: 39586682
DOI: 10.1101/pdb.prot108628

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