Article
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes.
Nature communications - 18 Jan 2017
Liang Zhen, Chen Kunling, Li Tingdong, Zhang Yi, Wang Yanpeng, Zhao Qian, Liu Jinxing, Zhang Huawei, Liu Cuimin, Ran Yidong, Gao Caixia
Abstract excerpt
Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop breeding. The avoidance of transgene integration and reduction of off-target mutations are the most important targets for optimization. Here, we describe an efficient genome editing method for bread wheat using CRISPR/Cas9 ribonucleoproteins (RNPs). Starting from RNP preparation, the whole protocol takes only seven to nine...
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