A Hierarchical Screening Strategy for Genome-Edited Events in Polyploid Species: A Case Study on Hexaploid Common Wheat.

Abstract excerpt

Genome editing via CRISPR/Cas9 has been widely adopted in cereal crops. In diploid species such as rice and barley, the generation of knockout mutants is relatively straightforward for functional characterization of the genes of interest due to their single-copy nature in the genome. In contrast, common wheat (Triticum aestivum L.) is a hexaploid species comprising three subgenomes (AABBDD); consequently, most...

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