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Article

Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe.

Biochemical and biophysical research communications - 7 Jun 2018

Rossi Franca, Morrone Castrese, Massarotti Alberto, Ferraris Davide Maria, Valenti Anna, Perugino Giuseppe, Miggiano Riccardo

Abstract excerpt

The self-labeling protein tags are robust and versatile tools for studying different molecular aspects of cell biology. In order to be suitable for a wide spectrum of experimental conditions, it is mandatory that these systems are stable after the fluorescent labeling reaction and do not alter the properties of the fusion partner. SsOGT-H5 is an engineered variant alkylguanine-DNA-alkyl-transferase (OGT) of the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29684348
DOI: 10.1016/j.bbrc.2018.04.139

Genes

OGT

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