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Article

Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability.

Journal of biotechnology - 20 Dec 2014

Béhar Ghislaine, Pacheco Sabino, Maillasson Mike, Mouratou Barbara, Pecorari Frédéric

Abstract excerpt

As a useful reagent for biotechnological applications, a scaffold protein needs to be as stable as possible to ensure longer lifetimes. We have developed archaeal extremophilic proteins from the “7 kDa DNA-binding” family as scaffolds to derive affinity proteins (Affitins). In this study, we evaluated a rational structure/sequence-guided approach to stabilize an Affitin derived from Sac7d by transferring its...

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PMID: 25450641
DOI: 10.1016/j.jbiotec.2014.10.006

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