Article
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma.
Nature neuroscience - 1 Oct 2017
Chow Ryan D, Guzman Christopher D, Wang Guangchuan, Schmidt Florian, Youngblood Mark W, Ye Lupeng, Errami Youssef, Dong Matthew B, Martinez Michael A, Zhang Sensen, Renauer Paul, Bilguvar Kaya, Gunel Murat, Sharp Phillip A, Zhang Feng, Platt Randall J, Chen Sidi
Abstract excerpt
A causative understanding of genetic factors that regulate glioblastoma pathogenesis is of central importance. Here we developed an adeno-associated virus-mediated, autochthonous genetic CRISPR screen in glioblastoma. Stereotaxic delivery of a virus library targeting genes commonly mutated in human cancers into the brains of conditional-Cas9 mice resulted in tumors that recapitulate human glioblastoma. Capture...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.