Article
Comparison of Immunohistochemistry and Direct Sequencing Methods for Identification of the BRAFV600E Mutation in Papillary Thyroid Carcinoma.
Annals of surgical oncology - 1 Jun 2018
Kim Jong-Kyu, Seong Chan Yong, Bae In Eui, Yi Jin Wook, Yu Hyeong Won, Kim Su-Jin, Won Jae-Kyung, Chai Young Jun, Choi June Young, Lee Kyu Eun
Abstract excerpt
BACKGROUND: BRAFV600E mutation is the most common somatic variant in papillary thyroid carcinoma (PTC) and is associated with aggressive prognostic factors. The conventional detection method for BRAF mutations is polymerase chain reaction followed by Sanger sequencing. Recently, an immunohistoche...
Topics
- Adult
- DNA, Neoplasm
- False Negative Reactions
- Female
- Humans
- Immunohistochemistry
- Lymphatic Metastasis
- Male
- Middle Aged
- Mutation
- Neoplasm Invasiveness
- Predictive Value of Tests
- Proto-Oncogene Proteins B-raf
- Retrospective Studies