Article
Characterizing alleles with large deletions using region specific extraction.
Human immunology - 1 Jun 2018
Steiner Noriko K, Hou Lihua, Hurley Carolyn Katovich
Abstract excerpt
Two novel HLA class II alleles, DRB4*03:01N and DQB1*03:276N, containing large deletions were identified during routine typing. Extraction of DNA encompassing the deletions was carried out with a panel of capture oligonucleotides followed by whole genome amplification. Next generation DNA sequencing was then used to characterize the sequences. DRB4*03:01N has a 16 kilobase pair deletion stretching upstream from...
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