Article
Long-range PCR amplification as an alternative strategy for characterizing novel HLA-B alleles.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Aug 1996
Curran M D, Williams F, Earle J A, Rima B K, van Dam M G, Bunce M, Middleton D
Abstract excerpt
We have developed a simple, rapid and reliable method for specifically amplifying and cloning full-length HLA-B genes from genomic DNA. Using this methodology we characterized three alleles of interest at the molecular level. Two of the alleles appeared in our routine class I PCR-SSOP typing syst...
Topics
- Alleles
- Base Sequence
- DNA
- Genetic Variation
- HLA-B Antigens
- HLA-B35 Antigen
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Homology, Nucleic Acid
- Tumor Cells, Cultured