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Article

Long-range PCR amplification as an alternative strategy for characterizing novel HLA-B alleles.

European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Aug 1996

Curran M D, Williams F, Earle J A, Rima B K, van Dam M G, Bunce M, Middleton D

Abstract excerpt

We have developed a simple, rapid and reliable method for specifically amplifying and cloning full-length HLA-B genes from genomic DNA. Using this methodology we characterized three alleles of interest at the molecular level. Two of the alleles appeared in our routine class I PCR-SSOP typing system, a variant of B*5801 found in the Daudi cell line and RCE 56 and a variant of B*4101 found in a number of volunteer...

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Identifiers

PMID: 8858286
DOI: 10.1111/j.1744-313x.1996.tb00125.x

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