Article
Genetic mapping of species differences via in vitro crosses in mouse embryonic stem cells.
Proceedings of the National Academy of Sciences of the United States of America - 3 Apr 2018
Lazzarano Stefano, Kučka Marek, Castro João P L, Naumann Ronald, Medina Paloma, Fletcher Michael N C, Wombacher Rebecka, Gribnau Joost, Hochepied Tino, Van Montagu Marc, Libert Claude, Chan Yingguang Frank
Abstract excerpt
Discovering the genetic changes underlying species differences is a central goal in evolutionary genetics. However, hybrid crosses between species in mammals often suffer from hybrid sterility, greatly complicating genetic mapping of trait variation across species. Here, we describe a simple, robust, and transgene-free technique to generate "in vitro crosses" in hybrid mouse embryonic stem (ES) cells by inducing...
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