Article
Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs.
Scientific reports - 26 Feb 2018
Sheets Timothy P, Park Ki-Eun, Park Chi-Hun, Swift Steven M, Powell Anne, Donovan David M, Telugu Bhanu P
Abstract excerpt
The domestic pig is an attractive model for biomedical research because of similarities in anatomy and physiology to humans. However, key gaps remain in our understanding of the role of developmental genes in pig, limiting its full potential. In this publication, the role of NEUROGENIN 3 (NGN3), a transcription factor involved in endocrine pancreas development has been investigated by CRISPR/Cas9 gene ablation....
Topics
- Animals
- Basic Helix-Loop-Helix Transcription Factors
- CRISPR-Associated Protein 9
- Endocrine Cells
- Female
- Gene Expression
- Gene Knockout Techniques
- Genotype
- Glucagon
- Insulin
- Islets of Langerhans