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Article

First efficient CRISPR-Cas9-mediated genome editing in L eishmania parasites

4 May 2015

Abstract excerpt

Protozoan pathogens that cause leishmaniasis in humans are relatively refractory to genetic manipulation. In this work, we implemented the CRISPR-Cas9 system in Leishmania parasites and demonstrated its efficient use for genome editing. The Cas9 endonuclease was expressed under the control of the Dihydrofolate Reductase-Thymidylate Synthase (DHFR-TS) promoter and the single guide RNA was produced under the...

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CRISPR and Genetic Engineering
Innovation and Socioeconomic Development
Research on Leishmaniasis Studies

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 25939677
DOI: 10.1111/cmi.12456

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