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Article

An RNA Interference (RNAi) Toolkit and Its Utility for Functional Genetic Analysis of Leishmania (Viannia).

Genes - 28 Dec 2022

Lye Lon-Fye, Owens Katherine L, Jang Soojin, Marcus Joseph E, Brettmann Erin A, Beverley Stephen M

Abstract excerpt

RNA interference (RNAi) is a powerful tool whose efficacy against a broad range of targets enables functional genetic tests individually or systematically. However, the RNAi pathway has been lost in evolution by a variety of eukaryotes including most Leishmania sp. RNAi was retained in species of the Leishmania subgenus Viannia, and here we describe the development, optimization, and application of RNAi tools to...

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PMID: 36672832
DOI: 10.3390/genes14010093
PMCID: PMC9858808

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