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An optimized targeted Next-Generation Sequencing approach for sensitive detection of single nucleotide variants

8 Jan 2018

Abstract excerpt

Monitoring of minimal residual disease (MRD) has become an important clinical aspect for early relapse detection during follow-up care after cancer treatment. Still, the sensitive detection of single base pair point mutations via Next-Generation Sequencing (NGS) is hampered mainly due to high substitution error rates. We evaluated the use of NGS for the detection of low-level variants on an Ion Torrent PGM...

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PMID: 29349042
DOI: 10.1016/j.bdq.2017.12.001

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