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Article

Affinity maturation of a portable Fab-RNA module for chaperone-assisted RNA crystallography.

Nucleic acids research - 16 Mar 2018

Koirala Deepak, Shelke Sandip A, Dupont Marcel, Ruiz Stormy, DasGupta Saurja, Bailey Lucas J, Benner Steven A, Piccirilli Joseph A

Abstract excerpt

Antibody fragments such as Fabs possess properties that can enhance protein and RNA crystallization and therefore can facilitate macromolecular structure determination. In particular, Fab BL3-6 binds to an AAACA RNA pentaloop closed by a GC pair with ∼100 nM affinity. The Fab and hairpin have served as a portable module for RNA crystallization. The potential for general application make it desirable to adjust the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29309709
DOI: 10.1093/nar/gkx1292
PMCID: PMC5861428

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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