Article
[Establishing the Ion Torrent PGM Sequencing Methods for the Clinical Diagnosis of MPN Patients].
Zhongguo shi yan xue ye xue za zhi - 1 Dec 2017
Huang Ji-Xian, Li Yu-Ling, Xu Na, Yin Chang-Xin, Zhou Xuan, Pan Cheng-Yun, He Bo-Lin, Lu Zi-Yuan, Liu Qi-Fa, Liu Xiao-Li
Abstract excerpt
OBJECTIVE: To investigate the feasibility and relibility of rapidly and accurately acquiring the informations of gene mutations in MPN patients by using self-designed custom MPN mutation-related multipe-PCR primer kit and next generation Ion Torrent PGM sequencing platform. METHODS: The bone marrow samples of 10 MPN patients with JAK2V617F and/or CALR+, Ph- confirmed by sanger sequencing method were collected and...
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