Article
Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle.
Scientific reports - 19 Dec 2017
Ikeda Mitsumi, Matsuyama Shuichi, Akagi Satoshi, Ohkoshi Katsuhiro, Nakamura Sho, Minabe Shiori, Kimura Koji, Hosoe Misa
Abstract excerpt
Isoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to create a double-strand break near the mutation site. CRISPR/Cas9 and donor DNA that contained a synonymous codon for the correct...
Topics
- Animals
- Blastocyst
- CRISPR-Cas Systems
- Cattle
- Cattle Diseases
- Cell Line
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA
- Endonucleases
- Gene Editing
- Genome
- Green Fluorescent Proteins
- Isoleucine-tRNA Ligase