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Article

Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle.

Scientific reports - 19 Dec 2017

Ikeda Mitsumi, Matsuyama Shuichi, Akagi Satoshi, Ohkoshi Katsuhiro, Nakamura Sho, Minabe Shiori, Kimura Koji, Hosoe Misa

Abstract excerpt

Isoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to create a double-strand break near the mutation site. CRISPR/Cas9 and donor DNA that contained a synonymous codon for the correct...

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Identifiers

PMID: 29259316
DOI: 10.1038/s41598-017-17968-w
PMCID: PMC5736618

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