Article
Establishment of protocol for preparation of gene-edited bovine ear-derived fibroblasts for somatic cell nuclear transplantation.
Biomedical research (Tokyo, Japan) - 1 Jan 2018
Ishino Takeshi, Hashimoto Mayuko, Amagasa Misato, Saito Natsuko, Dochi Osamu, Kirisawa Rikio, Kitamura Hiroshi
Abstract excerpt
Recently, gene-editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) technique has attempted to utilize fibroblasts of livestock animals for somatic cell nuclear transfer. In this study, we establish the procedure for preparing skin fibroblast clones whose genes were edited by the CRISPR/Cas9 technique. After isolating fibroblasts from earlobes of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.