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Article

Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Du Dijun, Wang Zhao, Chiu Wah, Luisi Ben F

Abstract excerpt

The cell envelope of Gram-negative bacteria comprises an outer membrane, a cytoplasmic inner membrane, and an interstitial space. The tripartite multidrug transporter AcrAB-TolC, which uses proton electrochemical gradients to vectorially drive the efflux of drugs from the cell, spans this envelope. We describe here details of the methods used to prepare the recombinant tripartite assembly for high-resolution...

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PMID: 29177826
DOI: 10.1007/978-1-4939-7454-2_5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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