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Article

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System.

Journal of visualized experiments : JoVE - 10 Mar 2020

Chetrit David, Park Donghyun, Hu Bo, Liu Jun, Roy Craig R

Abstract excerpt

The Dot/Icm secretion system of Legionella pneumophila is a complex type IV secretion system (T4SS) nanomachine that localizes at the bacterial pole and mediates the delivery of protein and DNA substrates to target cells, a process generally requiring direct cell-to-cell contact. We have recently solved the structure of the Dot/Icm apparatus by cryo-electron tomography (cryo-ET) and showed that it forms a cell...

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PMID: 32225141
DOI: 10.3791/60693

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