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Article

High-resolution landscape of an antibiotic binding site.

Nature - 1 Oct 2023

Yang Kevin B, Cameranesi Maria, Gowder Manjunath, Martinez Criseyda, Shamovsky Yosef, Epshtein Vitaliy, Hao Zhitai, Nguyen Thao, Nirenstein Eric, Shamovsky Ilya, Rasouly Aviram, Nudler Evgeny

Abstract excerpt

Antibiotic binding sites are located in important domains of essential enzymes and have been extensively studied in the context of resistance mutations; however, their study is limited by positive selection. Using multiplex genome engineering1 to overcome this constraint, we generate and characterize a collection of 760 single-residue mutants encompassing the entire rifampicin binding site of Escherichia coli RNA...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 37648864
DOI: 10.1038/s41586-023-06495-6
PMCID: PMC10550828

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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