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Article

Generation of Conditional Mutants in Borrelia burgdorferi.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Latham Jacob I, Blevins Jon S

Abstract excerpt

Mutational studies aimed at characterizing the function(s) of bacterial genes required for growth or viability are constrained by the inability to generate deletion strains lacking the gene of interest. To circumvent this limitation, it is possible to generate conditional mutants in which a copy of the gene of interest is introduced into the bacteria to compensate for the loss of the native allele. Expression of...

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PMID: 29032548
DOI: 10.1007/978-1-4939-7383-5_17

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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