Isolation and characterization of chemotaxis mutants of the Lyme disease Spirochete Borrelia burgdorferi using allelic exchange mutagenesis, flow cytometry, and cell tracking.

Abstract excerpt

Constructing mutants by targeted gene inactivation is more difficult in the Lyme disease organism, Borrelia burgdorferi, than in many other species of bacteria. The B. burgdorferi genome is fragmented, with a large linear genome and 21 linear and circular plasmids. Some of these small linear and circular plasmids are often lost during laboratory propagation, and the loss of specific plasmids can have a...

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