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Article

Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes.

MicrobiologyOpen - 1 Apr 2013

Bloodworth Ruhi A M, Gislason April S, Cardona Silvia T

Abstract excerpt

Identification of essential genes by construction of conditional knockouts with inducible promoters allows the identification of essential genes and creation of conditional growth (CG) mutants that are then available as genetic tools for further studies. We used large-scale transposon delivery of the rhamnose-inducible promoter, PrhaB followed by robotic screening of rhamnose-dependent growth to construct a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23389959
DOI: 10.1002/mbo3.71
PMCID: PMC3633349

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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