Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Reversed genetics: a new approach to the elucidation of structure--function relationship.

Ciba Foundation symposium - 1 Jan 2000

Weissmann C, Weber H, Taniguchi T, Müller W, Meyer F

Abstract excerpt

Methods for generating point mutations at predetermined sites of RNA or DNA genomes have been developed. With Qbeta RNA as a template, minus strands were synthesized in vitro in a stepwise, substrate-controlled reaction. The nucleotide analogue N4-hydorxyCMP was introduced in the desired position, the minus strands were completed with the four standard triphosphates and used as templates to synthesize plus...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Mutation
Share to Base Sequence
Share to Binding Sites

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Globins

Share to Plasmids

Share to RNA, Viral

Source

Identifiers

PMID: 289481
DOI: 10.1002/9780470720486.ch4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Reversed genetics: a new approach to the elucidation of structure--function relationship.PMID 289481Read ↗

cis-acting elements within an RNA coliphage genome: fold as you please, but fold you must!!Semantic proximity Read ↗

A site-directed mutagenesis method utilising large double-stranded DNA templates for the simultaneous introduction of multiple changes and sequential multiple rounds of mutation: Application to the study of whole viral genomes.Semantic proximity Read ↗

In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties.Semantic proximity Read ↗

Physical mapping of a collection of Mael-generating amber mutations in the beta gene of Escherichia coli RNA polymerase and the functional effect of internal deletions constructed through their manipulation.Semantic proximity Read ↗

A novel simple and rapid PCR-based site-directed mutagenesis method.Semantic proximity Read ↗

In vitro selection of second site revertants analysis of the hairpin ribozyme active site.Semantic proximity Read ↗

Point mutations in the ICR2 motif of brome mosaic virus RNAs debilitate (+)-strand replication.Semantic proximity Read ↗

Mutation in Escherichia coli B/r WP2 try by reversion or suppression of a chain-terminating codon.Semantic proximity Read ↗

Base Pairing between the 5′ Half of ε and a <i>cis</i> -Acting Sequence, Φ, Makes a Contribution to the Synthesis of Minus-Strand DNA for Human Hepatitis B VirusSemantic proximity Read ↗

Pseudorabies virus glycoprotein C attachment-proficient revertants isolated through a simple, targeted mutagenesis scheme.Semantic proximity Read ↗

Altered 3'-terminal RNA structure in phage Qbeta adapted to host factor-less Escherichia coli.Semantic proximity Read ↗

Structure-function relationships of the initiation complex of HIV-1 reverse transcription: the case of mutant viruses using tRNA(His) as primer.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.