Article
Reversed genetics: a new approach to the elucidation of structure--function relationship.
Ciba Foundation symposium - 1 Jan 2000
Weissmann C, Weber H, Taniguchi T, Müller W, Meyer F
Abstract excerpt
Methods for generating point mutations at predetermined sites of RNA or DNA genomes have been developed. With Qbeta RNA as a template, minus strands were synthesized in vitro in a stepwise, substrate-controlled reaction. The nucleotide analogue N4-hydorxyCMP was introduced in the desired position, the minus strands were completed with the four standard triphosphates and used as templates to synthesize plus...
Topics
- Base Sequence
- Binding Sites
- DNA
- DNA, Recombinant
- Genes
- Genetic Engineering
- Globins
- Mutation
- Plasmids
- RNA, Viral
- Ribosomes