Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Spirohexene-Tetrazine Ligation Enables Bioorthogonal Labeling of Class B G Protein-Coupled Receptors in Live Cells.

Journal of the American Chemical Society - 27 Sept 2017

Ramil Carlo P, Dong Maoqing, An Peng, Lewandowski Tracey M, Yu Zhipeng, Miller Laurence J, Lin Qing

Abstract excerpt

A new bioorthogonal reactant pair, spiro[2.3]hex-1-ene (Sph) and 3,6-di(2-pyridyl)-s-tetrazine (DpTz), for the strain-promoted inverse electron-demand Diels-Alder cycloaddition, that is, tetrazine ligation, is reported. As compared to the previously reported strained alkenes such as trans-cyclooctene (TCO) and 1,3-disubstituted cyclopropene, Sph exhibits balanced reactivity and stability in tetrazine ligation...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Survival
Share to Fluorescent Dyes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to HEK293 Cells

Share to Ligands

Share to Mutation

Share to Receptors, G-Protein-Coupled

Share to Spiro Compounds

Share to Staining and Labeling

Source

Open in PubMed Open full text

Identifiers

PMID: 28876923
DOI: 10.1021/jacs.7b05674
PMCID: PMC5753752

Genes

GCGR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Spirohexene-Tetrazine Ligation Enables Bioorthogonal Labeling of Class B G Protein-Coupled Receptors in Live Cells.PMID 28876923Read ↗

Semi-synthetic nanobody-ligand conjugates exhibit tunable signaling properties and enhanced transcriptional outputs at neurokinin receptor-1Semantic proximity Read ↗

G Protein–Coupled Receptors Revisited: Therapeutic Applications Inspired by Synthetic BiologySemantic proximity Read ↗

ChemCell: Chemical Tethering of Large Biomolecules to Cell Surfaces through Diels-Alder LigationSemantic proximity Read ↗

Strain-promoted stapled peptides for inhibiting protein-protein interactionsSemantic proximity Read ↗

DREADDs (designer receptors exclusively activated by designer drugs): chemogenetic tools with therapeutic utility.Semantic proximity Read ↗

Optical tools for visualizing and controlling human GLP-1 receptor activation with high spatiotemporal resolutionSemantic proximity Read ↗

Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor.Semantic proximity Read ↗

Blind De Novo Design of Dual Cyclic Peptide Agonists Targeting GCGR and GLP1RSemantic proximity Read ↗

Mapping the ligand-binding site on a G protein-coupled receptor (GPCR) using genetically encoded photocrosslinkers.Semantic proximity Read ↗

An enzymatic [4+2] cyclization cascade creates the pentacyclic core of pyrroindomycins.Semantic proximity Read ↗

1-(Aminomethyl)-Trans-Cyclooct-4-Ene, a New TCO Derivative for Target Visualization and Target Engagement Measurement in CellsSemantic proximity Read ↗

Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.