Article
A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.
Fungal genetics and biology : FG & B - 1 Nov 2017
Upadhya Rajendra, Lam Woei C, Maybruck Brian T, Donlin Maureen J, Chang Andrew L, Kayode Sarah, Ormerod Kate L, Fraser James A, Doering Tamara L, Lodge Jennifer K
Abstract excerpt
C. neoformans is an encapsulated fungal pathogen with defined asexual and sexual life cycles. Due to the availability of genetic and molecular tools for its manipulation, it has become a model organism for studies of fungal pathogens, even though it lacks a reliable system for maintaining DNA fragments as extrachromosomal plasmids. To compensate for this deficiency, we identified a genomic gene-free intergenic...
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