Improvement of extracellular lipase production by a newly isolated Yarrowia lipolytica mutant and its application in the biosynthesis of L-ascorbyl palmitate.

Abstract excerpt

Yarrowia lipolytica Wt-11 producing an extracellular lipase was isolated and identified. To improve the lipase production, Y. lipolytica Wt-11 was subjected to low-energy ion implantation mutation breeding, and a best mutant, Y. lipolytica Mut-96, was obtained after screening. Under the optimal cultivation conditions, the scaled-up production of lipases were performed, and the lipase activity of Y. lipolytica...

Topics

• Ascorbic Acid
• Culture Media
• Enzyme Stability
• Fermentation
• Fungal Proteins
• Gene Expression
• Hydrogen-Ion Concentration
• Kinetics
• Lipase
• Mutation
• Temperature