Article
High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate.
New biotechnology - 1 Oct 2011
Darvishi Farshad, Destain Jacqueline, Nahvi Iraj, Thonart Philippe, Zarkesh-Esfahani Hamid
Abstract excerpt
The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica treated with EMS...
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