Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of wild-type Yarrowia lipolytica IMUFRJ 50682 auxotrophic mutants using dual CRISPR/Cas9 strategy for novel biotechnological approaches.

Enzyme and microbial technology - 1 Oct 2020

de Souza Camilla Pires, Ribeiro Bernardo Dias, Zarur Coelho Maria Alice, Almeida Rodrigo Volcan, Nicaud Jean-Marc

Abstract excerpt

Yarrowia lipolytica IMUFRJ 50682 is a Brazilian wild-type strain with potential application in bioconversion processes which can be improved through synthetic biology. In this study, we focused on a combinatorial dual cleavage CRISPR/Cas9-mediated for construction of irreversible auxotrophic mutants IMUFRJ 50682, which genomic information is not available, thought paired sgRNAs targeting upstream and downstream...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to beta Carotene
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fluorescence

Share to Gene Targeting

Share to Metabolic Engineering

Share to RNA, Guide, CRISPR-Cas Systems

Share to Uracil

Share to Yarrowia

Source

Identifiers

PMID: 32912681
DOI: 10.1016/j.enzmictec.2020.109621

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of wild-type Yarrowia lipolytica IMUFRJ 50682 auxotrophic mutants using dual CRISPR/Cas9 strategy for novel biotechnological approaches.PMID 32912681Read ↗

A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.Semantic proximity Read ↗

Genome-wide CRISPR-Cas9 screen reveals a persistent null-hyphal phenotype that maintains high carotenoid production in Yarrowia lipolytica.Semantic proximity Read ↗

mpCRISTAR: Multiple Plasmid Approach for CRISPR/Cas9 and TAR-Mediated Multiplexed Refactoring of Natural Product Biosynthetic Gene Clusters.Semantic proximity Read ↗

Genetic Engineering in Bacteria, Fungi, and OomycetesSemantic proximity Read ↗

Multidimensional combinatorial screening for high-level production of erythritol in Yarrowia lipolytica.Semantic proximity Read ↗

CRISPR/Cas9 technology enables the development of the filamentous ascomycete fungus Penicillium subrubescens as a new industrial enzyme producer.Semantic proximity Read ↗

CRISPRi-Driven Genetic Screening for Designing Novel Microbial Phenotypes.Semantic proximity Read ↗

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production.Semantic proximity Read ↗

Comparative genomics analysis of a series of Yarrowia lipolytica WSH-Z06 mutants with varied capacity for α-ketoglutarate production.Semantic proximity Read ↗

Highly efficient genome editing using CRISPR/Cas9 ribonucleoprotein in the marine oleaginous diatom Fistulifera solaris.Semantic proximity Read ↗

Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods.Semantic proximity Read ↗

Biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening for over-production phenotypes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.