Article
Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients.
Clinical chemistry - 1 Dec 2013
Taly Valerie, Pekin Deniz, Benhaim Leonor, Kotsopoulos Steve K, Le Corre Delphine, Li Xinyu, Atochin Ivan, Link Darren R, Griffiths Andrew D, Pallier Karine, Blons Hélène, Bouché Olivier, Landi Bruno, Hutchison J Brian, Laurent-Puig Pierre
Abstract excerpt
BACKGROUND: Multiplex digital PCR (dPCR) enables noninvasive and sensitive detection of circulating tumor DNA with performance unachievable by current molecular-detection approaches. Furthermore, picodroplet dPCR facilitates simultaneous screening for multiple mutations from the same sample. METHODS: We investigated the utility of multiplex dPCR to screen for the 7 most common mutations in codons 12 and 13 of the...
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