Article
Next-generation sequencing (NGS)-based identification of induced mutations in a doubly mutagenized tomato (Solanum lycopersicum) population.
The Plant journal : for cell and molecular biology - 1 Nov 2017
Gupta Prateek, Reddaiah Bodanapu, Salava Hymavathi, Upadhyaya Pallawi, Tyagi Kamal, Sarma Supriya, Datta Sneha, Malhotra Bharti, Thomas Sherinmol, Sunkum Anusha, Devulapalli Sameera, Till Bradley John, Sreelakshmi Yellamaraju, Sharma Rameshwar
Abstract excerpt
The identification of mutations in targeted genes has been significantly simplified by the advent of TILLING (Targeting Induced Local Lesions In Genomes), speeding up the functional genomic analysis of animals and plants. Next-generation sequencing (NGS) is gradually replacing classical TILLING for mutation detection, as it allows the analysis of a large number of amplicons in short durations. The NGS approach...
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