Article
Genome-wide survey of artificial mutations induced by ethyl methanesulfonate and gamma rays in tomato.
Plant biotechnology journal - 1 Jan 2016
Shirasawa Kenta, Hirakawa Hideki, Nunome Tsukasa, Tabata Satoshi, Isobe Sachiko
Abstract excerpt
Genome-wide mutations induced by ethyl methanesulfonate (EMS) and gamma irradiation in the tomato Micro-Tom genome were identified by a whole-genome shotgun sequencing analysis to estimate the spectrum and distribution of whole-genome DNA mutations and the frequency of deleterious mutations. A total of ~370 Gb of paired-end reads for four EMS-induced mutants and three gamma-ray-irradiated lines as well as a...
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