Article
High-throughput detection of induced mutations and natural variation using KeyPoint technology.
PloS one - 1 Jan 2009
Rigola Diana, van Oeveren Jan, Janssen Antoine, Bonné Anita, Schneiders Harrie, van der Poel Hein J A, van Orsouw Nathalie J, Hogers René C J, de Both Michiel T J, van Eijk Michiel J T
Abstract excerpt
Reverse genetics approaches rely on the detection of sequence alterations in target genes to identify allelic variants among mutant or natural populations. Current (pre-) screening methods such as TILLING and EcoTILLING are based on the detection of single base mismatches in heteroduplexes using endonucleases such as CEL 1. However, there are drawbacks in the use of endonucleases due to their relatively poor...
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