Nucleolytic degradation of 3'-ending overhangs is essential for DNA-end resection in RecA-loading deficient recB mutants of Escherichia coli.

doi:10.1016/j.dnarep.2017.06.024

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Article

Nucleolytic degradation of 3'-ending overhangs is essential for DNA-end resection in RecA-loading deficient recB mutants of Escherichia coli.

DNA repair - 1 Sept 2017

Ivanković Siniša, Vujaklija Dušica, Đermić Damir

Abstract excerpt

Degradation of a 5'-ending strand is the hallmark of the universal process of DNA double strand break (DSB) resection, which results in creation of the central recombination intermediate, a 3'-ending overhang. Here we show that in Escherichia coli recB1080/recB1067 mutants, which are devoid of RecBCD's nuclease and RecA loading activities, degradation of the unwound 3' tail is as essential as is degradation of...

Topics

DNA Breaks, Double-Stranded
DNA, Bacterial
Escherichia coli
Escherichia coli Proteins
Exodeoxyribonuclease V
Exodeoxyribonucleases
Homologous Recombination
Mutation
Rec A Recombinases
RecQ Helicases
Recombinational DNA Repair

Source

Open in PubMed

Identifiers

PMID: 28689072
DOI: 10.1016/j.dnarep.2017.06.024

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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