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Article

Efficient identification of SNPs in pooled DNA samples using a dual mononucleotide addition-based sequencing method.

Molecular genetics and genomics : MGG - 1 Oct 2017

Cao Changchang, Pan Rongfang, Tan Jun, Sun Xiao, Xiao Pengfeng

Abstract excerpt

Identifying single nucleotide polymorphism (SNPs) from pooled samples is critical for many studies and applications. SNPs determined by next-generation sequencing results may suffer from errors in both base calling and read mapping. Taking advantage of dual mononucleotide addition-based pyrosequencing, we present Epds, a method to efficiently identify SNPs from pooled DNA samples. On the basis of only five...

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PMID: 28612167
DOI: 10.1007/s00438-017-1332-2
PMCID: PMC5594057

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