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Pyrosequencing with di-base addition for single nucleotide polymorphism genotyping.

Analytical and bioanalytical chemistry - 1 May 2016

Pu Dan, Mao Chengguang, Cui Lunbiao, Shi Zhiyang, Xiao Pengfeng

Abstract excerpt

We develop color code-based pyrosequencing with di-base addition for analysis of single nucleotide polymorphisms (SNPs). When a di-base is added into the polymerization, one or several two-color code(s) containing the type and the number of incorporated nucleotides will be produced. The code information obtained in a single run is useful to genotype SNPs as each allelic variant will give a specific pattern...

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PMID: 26935928
DOI: 10.1007/s00216-016-9359-5

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