Article
Comparative functional genomic screens of three yeast deletion collections reveal unexpected effects of genotype in response to diverse stress.
Open biology - 1 Jun 2017
Acton Erica, Lee Amy Huei-Yi, Zhao Pei Jun, Flibotte Stephane, Neira Mauricio, Sinha Sunita, Chiang Jennifer, Flaherty Patrick, Nislow Corey, Giaever Guri
Abstract excerpt
The Yeast Knockout (YKO) collection has provided a wealth of functional annotations from genome-wide screens. An unintended consequence is that 76% of gene annotations derive from one genotype. The nutritional auxotrophies in the YKO, in particular, have phenotypic consequences. To address this issue, 'prototrophic' versions of the YKO collection have been constructed, either by introducing a plasmid carrying...
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