Article
Genetic wiring maps of single-cell protein states reveal an off-switch for GPCR signalling.
Nature - 8 Jun 2017
Brockmann Markus, Blomen Vincent A, Nieuwenhuis Joppe, Stickel Elmer, Raaben Matthijs, Bleijerveld Onno B, Altelaar A F Maarten, Jae Lucas T, Brummelkamp Thijn R
Abstract excerpt
As key executers of biological functions, the activity and abundance of proteins are subjected to extensive regulation. Deciphering the genetic architecture underlying this regulation is critical for understanding cellular signalling events and responses to environmental cues. Using random mutagenesis in haploid human cells, we apply a sensitive approach to directly couple genomic mutations to protein...
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