Article
The first crystal structure of a family 129 glycoside hydrolase from a probiotic bacterium reveals critical residues and metal cofactors.
The Journal of biological chemistry - 21 Jul 2017
Sato Mayo, Liebschner Dorothee, Yamada Yusuke, Matsugaki Naohiro, Arakawa Takatoshi, Wills Siobhán S, Hattie Mitchell, Stubbs Keith A, Ito Tasuku, Senda Toshiya, Ashida Hisashi, Fushinobu Shinya
Abstract excerpt
The α-N-acetylgalactosaminidase from the probiotic bacterium Bifidobacterium bifidum (NagBb) belongs to the glycoside hydrolase family 129 and hydrolyzes the glycosidic bond of Tn-antigen (GalNAcα1-Ser/Thr). NagBb is involved in assimilation of O-glycans on mucin glycoproteins by B. bifidum in the human gastrointestinal tract, but its catalytic mechanism has remained elusive because of a lack of sequence homology...
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