Article
Functional and cooperative stabilization of a two-metal (Ca, Zn) center in α-amylase derived from Flavobacteriaceae species.
Scientific reports - 20 Dec 2017
Yin Huijia, Yang Zhou, Nie Xinyu, Li Shannan, Sun Xuyang, Gao Chao, Wang Zenghang, Zhou Guangming, Xu Ping, Yang Chunyu
Abstract excerpt
Mesophilic α-amylase from Flavobacteriaceae (FSA) is evolutionary closely related to thermophilic archaeal Pyrococcus furiosus α-amylase (PWA), but lacks the high thermostability, despite the conservation of most residues involved in the two-metal (Ca, Zn) binding center of PWA. In this study, a disulfide bond was introduced near the two-metal binding center of FSA (designated mutant EH-CC) and this modification...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.